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SRX4155551: WGS primary paper sludge DNA cloned into fosmids
1 ILLUMINA (Illumina MiSeq) run: 28,786 spots, 13M bases, 8.9Mb downloads

Design: DNA library creation and sequencing was done using plexwell high throughput technology by Seqwell Inc. The library was Illumina NGS sequencing compatible
Submitted by: University of British Columbia
Study: paper pulp sludge enviromental metagenome
show Abstracthide Abstract
This Bioproject describes sampling and consequent metagenomic analysis of the microbial community inhabiting primary wastewater sludge of a coastal BC paper mill. The metagenomic DNA was used for creating a fosmid library that was mined for carbohydrate active enzymes (cAZymes) using a high throughput functional screen and selected clones applied for consolidated bioprocess development. In-silico analysis of the metagenomic data using several bioinformatics pipelines, most importantly Metapathways, was also conducted to complement experimental findings. The metagenome was taxonomically binned and functionally compared to data from other biomass waste environments. This project establishes proof of concept of application of metagenomics to circular bioeconomy development.
Sample: Pulp and paper sludge metagenome sample from coastal BC mill
SAMN07843559 • SRS2849407 • All experiments • All runs
Library:
Name: PPSLIBM-31-H11
Instrument: Illumina MiSeq
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 28,786 spots, 13M bases, 8.9Mb
Run# of Spots# of BasesSizePublished
SRR725081928,78613M8.9Mb2019-07-01

ID:
5642177

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